Slime mold quiz: test your knowledge

Think you know slime mold? Take this 10-question quiz to find out how much you really know about Physarum polycephalum.

Question 1 / 10

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Every question, with its answer

The full answer key, question by question. Expand a question to see the correct answer and why.

1 What biological group does the blob (Physarum polycephalum) belong to?
  • Fungus
  • Bacterium
  • Myxomycete (protist)
  • Algae

Answer : Myxomycete (protist)

The blob is a myxomycete. It was long filed among the protists, a catch-all for organisms that are neither animals, plants nor fungi, and DNA now places it among the Amoebozoa. It is not a fungus, nor a bacterium, nor an alga.

2 How does slime mold move across surfaces?
  • Using tiny legs invisible to the naked eye
  • By rhythmic contractions of its cytoplasm (shuttle streaming)
  • By rolling like a ball
  • By growing roots that pull it forward

Answer : By rhythmic contractions of its cytoplasm (shuttle streaming)

Slime mold moves through cytoplasmic shuttle streaming: rhythmic back-and-forth contractions push the cytoplasm toward the leading edge. In ideal lab conditions it can advance up to about 4 cm an hour, closer to 1 cm an hour at home.

3 How did Audrey Dussutour demonstrate that slime mold has a form of memory?
  • By training it to recognize colors
  • By showing it could learn to ignore a repulsive but harmless substance
  • By teaching it to follow a light pattern
  • By conditioning it with electric shocks

Answer : By showing it could learn to ignore a repulsive but harmless substance

In 2016, Romain Boisseau, David Vogel and Audrey Dussutour (CNRS) showed that Physarum polycephalum learns to cross a bridge laced with quinine or caffeine once it has found the substance harmless. Later that year, Vogel and Dussutour showed that a slime mold trained this way could pass the behavior on to an untrained one by fusing with it.

4 How does slime mold reproduce sexually?
  • By budding, like yeast
  • By releasing spores that hatch into gamete cells which fuse
  • By binary fission
  • It cannot reproduce sexually

Answer : By releasing spores that hatch into gamete cells which fuse

Under stress, the plasmodium forms fruiting bodies that release spores. These spores hatch into myxamoebae or swarm cells, which then fuse in compatible pairs to form a new diploid plasmodium.

5 What was remarkable about the 2010 Tokyo railway experiment?
  • Slime mold built a network matching Tokyo's rail system
  • Slime mold survived Japanese winter outdoors
  • Slime mold consumed all food in record time
  • Slime mold refused to grow on the map

Answer : Slime mold built a network matching Tokyo's rail system

Researchers placed oat flakes on a map at the positions of the cities around Tokyo. The network the slime mold grew between them was comparable to the real rail system in cost, efficiency and resistance to breakdowns, showing it can solve a hard optimization problem without a brain.

6 What does slime mold primarily feed on in the wild?
  • Sunlight through photosynthesis
  • Bacteria, fungal spores, and yeasts
  • Minerals from the soil
  • Other slime molds

Answer : Bacteria, fungal spores, and yeasts

Slime mold feeds by phagocytosis, engulfing bacteria, fungal spores, yeasts, and other microorganisms. It plays an important ecological role as a decomposer in moist forest environments.

7 What is the largest verified size of a single Physarum polycephalum?
  • A few millimeters at most
  • About the size of a coin
  • No verified record exists: it grows as long as it is fed
  • Exactly one square meter

Answer : No verified record exists: it grows as long as it is fed

A plasmodium has no known size limit and roughly doubles its area every day when well fed. Figures of several square meters circulate online, but no size record has been measured and published in a verifiable way. In the wild, a few tens of centimeters is already a big one.

8 What is the maximum speed of slime mold movement under optimal conditions?
  • 0.5 cm/h
  • 4 cm/h
  • 20 cm/h
  • 1 m/h

Answer : 4 cm/h

Slime mold can advance up to about 4 cm per hour in ideal lab conditions on smooth agar. In a home culture, expect around 1 cm per hour. That is slow for us, but its whole body flows forward through its network.

9 What was the main purpose of the 2021 slime mold experiment aboard the International Space Station?
  • To test a new medicine on astronauts
  • An educational experiment run in parallel with thousands of French schools
  • To grow food for the crew
  • To prove that slime mold can live without water

Answer : An educational experiment run in parallel with thousands of French schools

In September 2021, Thomas Pesquet woke four slime molds aboard the ISS, while 4,500 French schools were invited to run the same experiment on Earth. All four revived and grew in orbit, but four specimens are too few for scientific conclusions, and the project was educational from the start.

10 How many mating types does Physarum polycephalum have?
  • 2 (male and female)
  • 4
  • Around 30
  • 720

Answer : 720

Mating type in Physarum polycephalum is set by three genes: matA (16 known versions), matB (15) and matC (3). That gives 16 × 15 × 3 = 720 combinations, so 720 mating types, and two reproductive cells can only pair if their combinations differ.